Untargeted metabolomics revealing changes in aroma substances in flue-cured tobacco

Abstract The composition and content of aroma substances in flue-cured tobacco ( Nicotiana tabacum L.) will affect the quality tobacco. To investigate correlation between various K326 before after flue-curing, their impact on diversity, this study employed middle leaves KRK26 as test materials. Samples were collected both flue-curing for untargeted metabolomics analysis. results showed that 584 metabolites significantly different there 44 aroma-related metabolites, including alcohols, aldehydes, phenols, organic acids, etc. analyzed compounds consist 37 known substances, while 7 previously not associated with aroma, have been identified other food products. These findings suggest these seven might may be potential compounds. Further analysis aldehydes increased but acids decreased. Furthermore, revealed a quantity type serve reference enhancing process optimizing industrial production cigarettes use cured leaves.